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We now show that activation of Cre by feeding a tamoxifen-containing diet induces an aplastic anemia affecting RBC as well as WBC not only in mice reconstituted with Gpx4 -deficient, but also in control mice with Gpx4 wt/wt ;CreERT2 BM cells thus confirming the acute toxic effect of Cre activation on the hematopoietic system described by Higashi et al .33 There is, however, a clear difference between mice harboring or lacking the Gpx4 gene in hematopoietic cells: mice reconstituted with Gpx4 wt/wt ;CreERT2 BM cells recovered within three to six weeks whereas mice reconstituted with cells lacking the Gpx4 gene failed to fully recover and remained partially anemic

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While gut microbiota depletion in control mice resulted in improved glucose tolerance, this effect was fully reversed upon a whole-body knockdown of the GLP-1 receptor ( Figures 2E, F ), suggesting that GLP-1 signaling is essential for the observed glucose tolerance improvement